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It also contains a guanine (G) with dimethylformamidyl (dmf) base protection
Vaccinia Capping Enzyme can add 7-methylguanylate cap structure (Cap 0) to the 5' end of RNA
50% Glyercol
providing a stable
and Uhlenbeck OC (1987) Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA template
mRNA Cap-2'-O-Methyltransferase RNases and inhibitors It also contains a guanineProduct Information mRNA Cap 2' O Methyltransferase is a recombinant protein from Vaccinia virus. The enzyme can add a methyl group at the 2' O position of the first nucleotide adjacent to the Cap structure at the 5' end of the RNA. The enzyme utilizes SAM as a methyl donor to methylate capped RNA (Cap 0) resulting in a cap 1 structure. The Cap 1 structure has been reported to enhance mRNA translation efficiency and hence may help improve expression
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