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1 Preparation of Standard Gradient Samples Reconstitute the lyophilized standard with 50μL deionized water
an intermediate domain containing the RIP homotypic interaction motif (RHIM)
dilution bufferOne copy of color developing solution (a
Thawed Cells: After thawing
Source Rat Synonym Rat Beclin-1 ELISA Kit Detection Type Double antibody sandwich method Composition Name 9 6 T match set remark Pre-coating 96 Well plate 8 Hole ×12 Strip without Standard 2 branch Dilute as per instructions
Streptavidin Donor Beads Cytokine & Cytokine Receptor 1 Preparation of Standard GradientProduct Specification Stability & Storage Store at 2 8C, protected from light; product shelf life is 12 months. Background Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads at close proximity, resulting in luminescence. Donor beads recognize Protein 1 (Tag1 label), while acceptor beads recognize Protein 2 (Tag2 label). When Protein 1 binds to Protein 2,