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20 units of SUMO protease can be used per 40μg of target protein for 1 hour at 30 °C
Fast Ligation Buffer should be thawed in advance at room temperature and mixed well by shaking up and down
See the figure below for details
NF-L is released into the cerebrospinal fluid (CSF) or blood
11 Add secondary antibody: The slides were washed 3 times by shaking in PBS (PH7
Rat CXCR3 ELISA Kit Cytokine & Cytokine Receptor 20 units of SUMO proteaseProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20