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TMB chromogenic substrate was added and incubated in the dark at room temperature for color development
and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation
0156 250 μL 250 μL of F solution H 0 250 μL /
MTT solvent (5ml)
and the substrate is subjected to color reaction in HRP It changed from colorless to blue under the catalysis of
CEA Recombinant Rabbit mAb (SDT-098-61) Secondary Antibody TMB chromogenic substrate was addedProduct Specification Host Rabbit Antigen CEA Synonyms Carcinoembryonic antigen related cell adhesion molecule 5, Carcinoembryonic antigen, CD66e Immunogen Recombinant Protein Location Secreted, Membrane Accession P06731 Clone Number SDT 098 61 Antibody Type Rabbit mAb Application Sandwich ELISA Reactivity Hu Cross Reactivity Does not recognize CEACAM 1, CEACAM 6, CEACAM 8 Purification Protein A Concentration 2 mg ml Purity >95% by HPLC Physical
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