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the cells were replaced with fresh culture medium preheated at 37℃ and incubated at 37℃ in the dark for another 30min to ensure that the intracellular esterase could fully hydrolyze Calcein AM to produce Calcein with green fluorescence
The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance
with C-terminal 10*His FPVSSKEENTKIVQDYLEKFYQLPSNQYQSTRKNGTSMIVEKLKEMQRFFGLNVTGKPNEETLDIMKKPRCGVPDSGDFMLTPGNPKWERTNLTYSILNYTPQLSETDVEGAIKKAFEVWSKASPLTFTRISQGQADINIAFFQRDHGDNSPFDGPNGILAHAFQPGQGIGGDAHFDAEETWTKNSTNYNLFLVAAHEFGHSLGLAHSSDPGALMYPNYAFRETSNYSLPQDDINGIQAIYGPSSNPIQPTGPTTPKSCDPRLTFDAITTLRGEILFFKDEYFWRRHPHLQSVEMNFISLFWPSLPTGIQAAYEDFDRDLIFLFKGNQYWAVSGYDIQQGYPRDISNYGFPSSVQAIDAAVFYRSKTYFFVNDQFWRYDNQRQFMEPGYPKSISGTFPGIENKVDAVFQQEHFFLFFSGPRYYAFDLIAERVTRVARANKWLNCRYSGGGSGGGSHHHHHHHHHH Expression System HEK293 Molecular Weight 65-75kDa Purity >95% by SDS-PAGE Endotoxin <0
N- and C-terminal domains required for protein-protein interactions
Purity >95% by SDS-PAGE & SEC-HPLC Endotoxin <0
CLEC12A/MICL/CLL-1 His Tag Protein, Mouse Kit the cells were replaced withProduct Specification Species Mouse Synonyms CLEC12A, MICL, CLL 1, CLL1, DCAL2, DCAL 2, CD371 Accession Q504P2 1 Amino Acid Sequence Tyr65 Arg267, with N terminal 8*His HHHHHHHHGGGSYTTLATEMIKSNQLQRAKEELQENVFLQLKHNLNSSKKIKNLSAMLQSTATQLCRELYSKEPEHKCKPCPKGSEWYKDSCYSQLNQYGTWQESVMACSARNASLLKVKNKDVLEFIKYKKLRYFWLALLPRKDRTQYPLSEKMFLSEESERSTDDIDKKYCGYIDRVNVYYTYCTDENNIICEETASKVQLESVLNGLPEDSR Expression System HEK293 Molecular Weight 28 40kDa Purity >95% by SDS
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