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The viability after resuscitation may decrease by more than 40%
it is required to "carry out subsequent experiments immediately"
The impact on cell viability is minimal: experimental data shows that the difference in cell viability after dissociating mouse skin with the two media is ≤5%
leading to the loss of target cells
Q: Both Step 3 and the incubation of Dissociation Solution 2 require a "37°C water bath or hybridization oven"
Dead Cell Removal Solution (DCRS) | Single-Cell Suspension Cleanup Viral RNA and DNA Extraction The viability after resuscitation mayFireGene Dead Cell Removal Solution is designed for removing dead cells from single cell suspensions derived from dissociated mammalian tissues. The workflow helps reduce dead cell contamination and improve sample background for downstream experiments such as flow cytometry analysis, single cell sequencing, cell culture, and related cell based assays. The protocol uses a layered separation workflow with DCRS, followed by centrifugation, collection of
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